ZDF Rat DiabeticBrain Microvascular Endothelial Cells

Catalog No.RD-6023

Suggested Medium:Endothelial Cell Medium /w Kit – 500 ml

Catalog No.M1266

Product Description

ZDF Rat Diabetic Brain Microvascular Endothelial Cells fromCell Biologicsare isolated from brain tissue of male, 9 week old laboratory ZDF rat.Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics’ Culture Complete Growth Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶cells per ml and is delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 (CD31) antibody, following the application of magnetic beads pre-coated with secondary antibody.

Product Testing

ZDF Rat DiabeticBrain MicrovascularEndothelial Cells are tested for expression of markers using antibody, PECAM-1 Antibody (M-20, sc-1506, Santa Cruz) or ZO-1 Rabbit Polyclonal Antibody (Catalog No. 617300, Life Technologies) by immunofluorescence staining. Cells are also tested for uptake of Dil-Ac-LDL (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified byCell Biologics.Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

ZDF Rat DiabeticBrain MicrovascularEndothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, transendothelial resistance (TER). Standard biochemical procedures performed with cell cultures include RT-PCR, western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage ofCell BiologicsProducts

Cell Biologicsships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen(-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Authorized Uses ofCell Biologics’Products

ZDF Rat DiabeticBrain MicrovascularEndothelial Cells fromCell Biologicsare distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of anyCell Biologics’cells or products from the purchaser to other markets, organizations or individuals is prohibited byCell Biologics,without the company’s written consent.Cell Biologics’Terms and Conditions must be accepted before submitting an order.

Disclaimer

Investigators should handle the cells that they receive fromCell Biologicswith caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Warranty and Liability

Cell Biologics’guarantee applies only to your purchase ofCell Biologics’cells withCell Biologics’Media and Coating Solution, for appropriate cell culture and cell testing followingCell Biologics’online protocols within 35 days from the date of product delivery.