CanineLiverKupfferCells
CatalogNo.D-6226
SuggestedMedium: MacrophageMedium/wKit(500ml)
CatalogNo.M3368
ProductDescription
CanineLiverKupfferCellsarederivedfromthelivertissueofbeagledog.CellsaregrownintissuecultureflasksandincubatedinCellBIOLOGics’CellCultureMediumfor2-5days.Priortoshipping,cellsatpassage0aredetachedfromflasksandcryopreservedinvials.Eachvialcontains3x106cellsandisdeliveredfrozen.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.CellsaretestedforexpressionofMarkersusingantibodies,CD11bbyflowcytometry.CellscanbeexpandedonamultiwellcultureplatereadyforexperimentsunderthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CanineLiverKupfferCellscanbeusedinstandardbiochemicalproceduresincludePCR,Westernblotting,immunoprecipitation,ROS,orcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicswillshipfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogenuntilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
CanineLiverKupfferCellsfromCellBiologicsaredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics'CellsorProductsfromthepurchasertoothermarkets,organizations,orindividualsisprohibitedbyCellBiologics.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Investigatorsshouldhandlethe
CellsthattheyreceivefromCellBiologicswithcautionandtreatallCellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat
http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.