CanineBoneMarrowMesenchymalStemCells(DBMMSCs)
Cataloguenumber:D-6043
SuggestedMedium:MesenchymalCellMedium/wKit–500ML
CatalogNo.M5566
ProductDescription
CanineBoneMarrowMesenchymalStemCellsarederivedfromthetibiasofbeagledog.CellsaregrownintissuecultureplateswithCellBIOLOGics’CultureMediumfor7-15days.Culturesarethenexpanded.Priortoshipping,cellsatpassage1aredetachedandcryo-preservedinvials.Eachvialcontains2x106cellspermlandisdeliveredfrozen.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor2or3passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
CanineBoneMarrowMesenchymalStemCellsaretestedforexpressionofMarkersusingantibodies,CD44,Sca-1andCD29byflowcytometry.
LaboratoryApplications
CanineBoneMarrowMesenchymalStemCellscanbeusedinstandardbiochemicalproceduresincludePCR,Westernblotting,immunoprecipitation,orcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice. Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Live-cellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
CanineBoneMarrowMesenchymalStemCellsfromCellBiologicsaredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologics,withoutthecompany’swrittenconsent. CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallCellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
