CaninePrimaryBrainVascularSmoothMuscleCells
CatalogNo. D-6085
SuggestedMedium: SmoothMuscleCellMedium/wKit–500ML
CatalogNo. M2268
ProductDescription
CaninePrimaryBrainVascularSmoothMuscleCellsfromCellBIOLOGicsareisolatedfrombraintissueofbeagledog.CaninePrimaryBrainVascularSmoothMuscleCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologicsCultureCompleteGrowthMediumgenerallyfor3-7days.Priortoshipping,cellsatpassage1aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106cellspermlandisdeliveredfrozen.Thecellsarecharacterizedbyimmunofluorescentmethodwithantibodiestoα-smoothmuscleactin.CaninePrimaryBrainVascularSmoothMuscleCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.CellscanbeexpandedonamultiwellcultureplatereadyforexperimentsunderthecellcultureconditionsspecifiedbyCellBiologics(Cellsmaybeexpandedfor2or3passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics).Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CaninePrimaryBrainVascularSmoothMuscleCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
CaninePrimaryBrainVascularSmoothMuscleCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
AlthoughCaninePrimaryBrainVascularSmoothMuscleCellsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
