CaninePrimaryAlveolarEpithelialCells
CatalogNo.D-6053
SuggestedMedium: EpithelialCellMedium/wKit–500ml
CatalogNo.H6621
ProductDescription
CaninePrimaryAlveolarEpithelialCellsfromCellBIOLOGicsareisolatedfromalveolartissueofbeagledog.CaninePrimaryAlveolarEpithelialCellsaregrowninaT25tissuecultureflaskpre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumfor3-5days.Priortoshipping,cellsatpassage2aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106cellspermlandisdeliveredfrozen.Themethodweusetoisolateprimaryepithelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.
CaninePrimaryAlveolarEpithelialCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CaninePrimaryAlveolarEpithelialCellscanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest. Primarycellsshouldneverbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
CaninePrimaryAlveolarEpithelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
