PorcineHepaticStellateCells
CatalogNo. P-6242
SuggestedMedium:CompleteHepaticStellateCellMedium/wKit–500ml
CatalogNo. M5569
ProductDescription
PorcinePrimaryHepaticStellateCellsfrom CellBIOLOGics areisolatedfromporcinelivertissue.Cellsaregrownintissuecultureflasksandincubatedin CellBiologics’ CellCultureMediumfor2-5days.Priortoshipping,cellsatpassage0aredetachedfromflasksandcryopreservedinvials. Eachvialcontains0.5x106 cellspermlandisdeliveredfrozen.
ProductTesting
PorcinePrimaryHepaticStellateCellsfrom CellBiologics aretestedforexpressionofMarkersusingantibodies,Desminorα-actinbyImmunofluorescenceStaining.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedonamultiwellcultureplatereadyforexperimentsunderthecellcultureconditionsspecifiedby CellBiologics.HepaticStellateCellsarenotrecommendforexpandingorlongtermculturessincethesecellswoulddifferentiatetobecomefibroblat-likecellsimmediatelyafterplatingandtheywillnotproliferateinculture.
LaboratoryApplications
PorcinePrimaryHepaticStellateCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogenuntilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.
AuthorizedUsesof CellBiologics Products
PorcinePrimaryHepaticStellateCellsfrom CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,for invitro diagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics, withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.InvestigatorsshouldhandletheCellsthattheyreceivefrom CellBiologics withcautionandtreatallCellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat
http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
