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Cell Biologics/Porcine Primary Dermal Epithelial Cells/P-6066/ T25 Fla
PorcinePrimary Dermal EpithelialCells
CatalogNo. P-6066
SuggestedMedium: EpithelialCellMedium/wKit–500ml
CatalogNo. H6621
ProductDescription
PorcinePrimaryDermalEpithelialCellsfromCellBIOLOGicsareisolatedfromskintissueofporcine.PorcinePrimaryDermalEpithelialCellsaregrowninaT25tissuecultureflaskpre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumfor3-5days.Cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106cellspermlandisdeliveredfrozen.Themethodweusetoisolateprimaryepithelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithEpCAM-1(CD326)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
PorcinePrimaryDermalEpithelialCellsarecharacterizedbyimmunofluorescentstainingwithantibodiesofE-cadherinorZO-1.PorcinePrimaryDermalEpithelialCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
PorcinePrimaryDermalEpithelialCellscanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
PorcinePrimaryDermalEpithelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
