C57BL/6-GFPMousePrimaryEmbryonicFibroblasts
CatalogNo.mGFP-6028
(CustomOrder,3-4weeks)
SuggestedMedium: FibroblastMedium/wKit-500ml
CatalogNo.M2267
ProductDescription
C57BL/6-GFPMousePrimaryEmbryonicFibroblastsfromCellBIOLOGicsareisolatedfromC57BL/6-Tg(CAG-EGFP)1Osb/Jmouseembryonictissueofpathogen-freelaboratorymice.C57BL/6-GFPMousePrimaryEmbryonicFibroblastsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologicsCultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded. Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106cellspermlandisdeliveredfrozen.
ProductTesting
C57BL/6-GFPMousePrimaryEmbryonicFibroblastsaretestedforexpressionofMarkerusingtheantibodyofanti-FSP1/S100A4(MilliporeUSA)byimmunofluorescencestaining.C57BL/6-GFPMousePrimaryEmbryonicFibroblastsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
C57BL/6-GFPMousePrimaryEmbryonicFibroblastscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest. Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
C57BL/6-GFPMousePrimaryEmbryonicFibroblastsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
AlthoughC57BL/6-GFPMouseEmbryonicFibroblastsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
