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Cell Biologics/C57BL/6-GFP Mouse Primary Cardiac Fibroblasts/mGFP-6049/
C57BL/6-GFPMousePrimaryCardiacFibroblasts
CatalogNo.mGFP-6049
SuggestedMedium: FibroblastMedium/wKit-500ml
CatalogNo.M2267
ProductDescription
C57BL/6-GFPMousePrimaryCardiacFibroblastsfromCellBIOLOGicsareisolatedfromC57BL/6-Tg(CAG-EGFP)1Osb/Jmousehearttissueofpathogen-freelaboratorymice.C57BL/6-GFPMousePrimaryCardiacFibroblastsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologicsCultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded. Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106cellspermlandisdeliveredfrozen.
ProductTesting
C57BL/6-GFPMousePrimaryCardiacFibroblastsaretestedforexpressionofMarkerusingtheantibodyofanti-FSP1/S100A4(MilliporeUSA)byimmunofluorescencestaining.C57BL/6-GFPMousePrimaryCardiacFibroblastsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
C57BL/6-GFPMousePrimaryCardiacFibroblastscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest. Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
C57BL/6-GFPMousePrimaryCardiacFibroblastsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
AlthoughC57BL/6-GFPMouseCardiacFibroblastsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
