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Cell Biologics/C57BL/6 Mouse Embryonic Aortic Smooth Muscle Cells/C57-6080E/
C57BL/6MouseEmbryonicAorticSmoothMuscleCells
CatalogNo. C57-6080E
SuggestedMedium: SmoothMuscleCellMedium/wKit–500ML
CatalogNo. M2268
ProductDescription
C57BL/6MouseEmbryonicAorticSmoothMuscleCellsfrom CellBIOLOGics areisolatedfromaortaofpathogen-freelaboratorymice atembryonicday14.5-19.CellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics CultureCompleteGrowthMediumgenerallyfor3-7days.Priortoshipping,cellsatpassage1aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106 cellspermlandisdeliveredfrozen.Thecellsarecharacterizedbyimmunofluorescentmethodwithantibodiestoα-smoothmuscleactin.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedonamultiwellcultureplatereadyforexperimentsunderthecellcultureconditionsspecifiedby CellBiologics (Cellsmaybeexpandedfor2or3passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics). Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
C57BL/6Mouse Embryonic AorticSmoothMuscleCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.
AuthorizedUsesof CellBiologics Products
C57BL/6Mouse Embryonic AorticSmoothMuscleCellsfrom CellBiologics aredistributedfor internal researchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics， withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
