C57BL/6-GFPMousePrimarySmallIntestinalMicrovascularEndothelialCells
CatalogNo.mGFP-6054
SuggestedMedium: EndothelialCellMedium/wKit–500ml
CatalogNo.M1168
ProductDescription
C57BL/6-GFPMousePrimarySmallIntestinalMicrovascularEndothelialCellsfromCellBIOLOGicsareisolatedfromC57BL/6-Tg(CAG-EGFP)1Osb/Jmousesmallintestinaltissueofpathogen-freelaboratorymice.C57BL/6-GFPMousePrimarySmallIntestinalMicrovascularEndothelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106cellspermlandaredeliveredfrozen.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticpre-coatedwithsecondaryantibody.
ProductTesting
C57BL/6-GFPMousePrimarySmallIntestinalMicrovascularEndothelialCellsaretestedforexpressionofMarkersusingantibody,VE-cadherin(CD144,VE-cadherinAntibody,C-19,sc6458,SantaCruz);AF1002(R&DSystem)orCD31/PECAM-1(PurifiedRatAnti-MouseCD31,CatalogNo.553370,BD)byimmunofluorescencestainingorFACS.C57BL/6-GFPMousePrimarySmallIntestinalMicrovascularEndothelialCellsarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-6passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
C57BL/6-GFPMousePrimarySmallIntestinalMicrovascularEndothelialCellscanbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,ortransendothelialresistance(TER).StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
C57BL/6-GFPMousePrimarySmallIntestinalMicrovascularEndothelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
AlthoughC57BL/6-GFPMousePrimarySmallIntestinalMicrovascularEndothelialCellsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
