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Cell Biologics/Cynomolgus Monkey Hepatic Stellate Cells/MK-6242/ Froze
CynomolgusMonkeyHepaticStellateCells
CatalogNo.MK-6242
SuggestedMedium:CompleteHepaticStellateCellMedium/wKit–500ml
CatalogNo.M5569
ProductDescription
CynomolgusMonkeyPrimaryHepaticStellateCellsfromCellBIOLOGicsareisolatedfromCynomolgusMonkeylivertissue.CellsaregrownintissuecultureflasksandincubatedinCellBiologics’CellCultureMediumfor2-5days.Priortoshipping,cellsatpassage0aredetachedfromflasksandcryopreservedinvials.Eachvialcontains0.5x106cellspermlandisdeliveredfrozen.
ProductTesting
CynomolgusMonkeyPrimaryHepaticStellateCellsfromCellBiologicsaretestedforexpressionofMarkersusingantibodies,Desminorα-actinbyImmunofluorescenceStaining.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.CellscanbeexpandedonamultiwellcultureplatereadyforexperimentsunderthecellcultureconditionsspecifiedbyCellBiologics.HepaticStellateCellsarenotrecommendforexpandingorlongtermculturessincethesecellswoulddifferentiatetobecomefibroblat-likecellsimmediatelyafterplatingandtheywillnotproliferateinculture.
LaboratoryApplications
CynomolgusMonkeyPrimaryHepaticStellateCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogenuntilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
CynomolgusMonkeyPrimaryHepaticStellateCellsfromCellBiologicsaredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologics,withoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.InvestigatorsshouldhandletheCellsthattheyreceivefromCellBiologicswithcautionandtreatallCellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat
http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
