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Cell Biologics/Cynomolgus Monkey Primary Coronary Artery Smooth Muscle Cells/MK-6001/
CynomolgusMonkeyPrimaryCoronaryArterySmoothMuscleCells
CatalogNo. MK-6001
SuggestedMedium: M2268SmoothMuscleCellMedium/wKit–500ML
ProductDescription
CynomolgusMonkeyPrimaryCoronaryArterySmoothMuscleCellsfromCellBIOLOGicsareisolatedfromtissueof CynomolgusMonkey coronaryartery.CynomolgusMonkeyPrimaryCoronaryArterySmoothMuscleCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologicsCultureCompleteGrowthMediumgenerallyfor3-7days.Priortoshipping,cellsatpassage1aredetachedfromthecultureflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106cellspermlandisdeliveredfrozen.Thecellsarecharacterizedbyimmunofluorescentmethodwithantibodiestoα-smoothmuscleactin.CynomolgusMonkeyPrimaryCoronaryArterySmoothMuscleCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedonamultiwellcultureplatereadyforexperiments(orcellsmaybeexpandedfor2passagesatasplitratioof1:2)underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CynomolgusMonkeyPrimaryCoronaryArterySmoothMuscleCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
CynomolgusMonkeyPrimaryCoronaryArterySmoothMuscleCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughMonkeyPrimaryCoronaryArterySmoothMuscleCellsareisolatedfromnormalmonkeytissue,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
