>
产品中心 >
acid_base_buffer_solution >
Cell Biologics/Cynomolgus Monkey Primary Liver Fibroblasts/MK-6019/ T7
CynomolgusMonkeyPrimaryLiverFibroblasts
CatalogNo. MK-6019
SuggestedMedium: FibroblastMedium/wKit–500ml
CaltalogNo.M2267
ProductDescription
CynomolgusMonkeyPrimaryLiverFibroblastsfromCellBIOLOGicsareisolatedfromnormal CynomolgusMonkey livertissue.CynomolgusMonkeyPrimaryLiverFibroblastsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage1aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106cellsperml.
ProductTesting
CynomolgusMonkeyPrimaryLiverFibroblastsfromCellBiologicsaretestedfornegativeexpressionofvonWillebrandFactorExpression/FactorVIII,cytokeratins18,andalphasmoothmuscleactin.CynomolgusMonkeyPrimaryLiverFibroblastsarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CynomolgusMonkeyPrimaryLiverFibroblastscanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Livecellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
CynomolgusMonkeyPrimaryLiverFibroblastsfromCellBiologicsaredistributedforlaboratoryresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,notforinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughCynomolgusMonkeyPrimaryLiverFibroblastsareisolatedfromnormalmonkeytissue,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
