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Cell Biologics/Cynomolgus Monkey Primary Coronary Artery Fibroblasts/MK-6049CO/
CynomolgusMonkey Primary CoronaryArteryFibroblasts
CatalogNo. MK-6049
SuggestedMedium: FibroblastMedium/wKit–500ml
CaltalogNo. M2267
ProductDescription
CynomolgusMonkeyPrimaryCoronaryArteryFibroblastsfrom CellBIOLOGics areisolatedfromnormal CynomolgusMonkey coronaryarterytissue.CynomolgusMonkeyPrimaryCoronaryArteryFibroblastsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’ CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage1aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106 cellsperml.
ProductTesting
CynomolgusMonkeyPrimaryCoronaryArteryFibroblastsfrom CellBiologics aretestedfornegativeexpressionofvonWillebrandFactorExpression/FactorVIII,cytokeratins18,andalphasmoothmuscleactin.CynomolgusMonkeyPrimaryCoronaryArteryFibroblastsarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CynomolgusMonkeyPrimaryCoronaryArteryFibroblastscanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Livecellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesof CellBiologics’Products
CynomolgusMonkeyPrimaryCoronaryArteryFibroblastsfrom CellBiologics aredistributedforlaboratoryresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,notforinvitrodiagnosticproceduresorfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughCynomolgusMonkeyPrimaryCoronaryArteryFibroblastsareisolatedfromnormalmonkeytissue,investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
