CynomolgusMonkey Primary SmallIntestinal EpithelialCells
CatalogNo. MK-6051
SuggestedMedium: EpithelialCellMedium/wKit–500ml
CalalogNo. H6621
ProductDescription
CynomolgusMonkeyPrimarySmallIntestinalEpithelialCellsfrom CellBIOLOGics areisolatedfromnormalCynomolgusMonkeysmallIntestinaltissue.CynomolgusMonkeyPrimarySmallIntestinalEpithelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’ CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106 cellsperml.Themethodweusetoisolateprimaryepithelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.CellsareincubatedwithEpCAM-1(CD326)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
CynomolgusMonkeyPrimarySmallIntestinalEpithelialCellsfrom CellBiologics aretestedforexpressionofMarkersusingantibody,E-cadherin(CatalogNo.610182BD)orZO-1RabbitPolyclonalAntibody(CatalogNo.617300;LifeTechnologies)byimmunofluorescencestaining.CynomolgusMonkeyPrimarySmallIntestinalEpithelialCellsarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CynomolgusMonkeyPrimarySmallIntestinalEpithelialCellscanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics’Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesof CellBiologics’Products
CynomolgusMonkeyPrimarySmallIntestinalEpithelialCellsfrom CellBiologics aredistributedforlaboratoryresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,notforinvitrodiagnosticproceduresorfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent.CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughCynomolgusMonkeyPrimarySmallIntestinalEpithelialCellsareisolatedfromnormalmonkeytissues,investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
