CynomolgusMonkey Primary EpidermalKeratinocytes
CatalogNo. MK-6066K
SuggestedMedium: EpithelialCellMedium/wKit–500ml
CalalogNo. H6621
ProductDescription
CynomolgusMonkeyPrimaryEpidermalKeratinocytesfrom CellBIOLOGics areisolatedfromnormalCynomolgusMonkeydermaltissue.CynomolgusMonkeyPrimaryEpidermalKeratinocytesaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’ CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106 cellsperml.
ProductTesting
CynomolgusMonkeyPrimaryEpidermalKeratinocytesfrom CellBiologics aretestedforexpressionofMarkersusingantibody,cytokeratine-18,-19or-8byimmunofluorescencestaining.CynomolgusMonkeyPrimaryEpidermalKeratinocytesarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CynomolgusMonkeyPrimaryEpidermalKeratinocytescanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics’Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Livecellshipmentisalsoavailableonrequest.Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesof CellBiologics’Products
CynomolgusMonkeyPrimaryEpidermalKeratinocytesfrom CellBiologics aredistributedforlaboratoryresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,notforinvitrodiagnosticproceduresorfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughCynomolgusMonkeyPrimaryEpidermalKeratinocytesareisolatedfromnormalmonkeytissues,investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
