CynomolgusMonkey Primary Alveolar EpithelialCells
CatalogNo. MK-6053
SuggestedMedium: EpithelialCellMedium/wKit–500ml
CalalogNo. H6621
ProductDescription
CynomolgusMonkeyPrimaryAlveolarEpithelialCellsfrom CellBIOLOGics areisolatedfromnormalCynomolgusMonkeylungtissue.CynomolgusMonkeyPrimaryAlveolarEpithelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’ CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106 cellsperml.Themethodweusetoisolateprimaryepithelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.CellsareincubatedwithEpCAM-1(CD326)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
CynomolgusMonkeyPrimaryAlveolarEpithelialCellsfrom CellBiologics aretestedforexpressionofMarkersusingantibody,E-cadherin(CatalogNo.610182BD)orZO-1RabbitPolyclonalAntibody(CatalogNo.617300;LifeTechnologies)byimmunofluorescencestaining.CynomolgusMonkeyPrimaryAlveolarEpithelialCellsarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor 3-7 passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CynomolgusMonkeyPrimaryAlveolarEpithelialCellscanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics’Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Livecellshipmentisalsoavailableonrequest.Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesof CellBiologics’Products
CynomolgusMonkeyPrimaryAlveolarEpithelialCellsfrom CellBiologics aredistributedforlaboratoryresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,notforinvitrodiagnosticproceduresorfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughCynomolgusMonkeyPrimaryAlveolarEpithelialCellsareisolatedfromnormalmonkeytissues,investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
