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Cell Biologics/Cynomolgus Monkey Primary Thyroid Microvascular Endothelial Cells/MK-6058/
CynomolgusMonkeyPrimaryThyroidMicrovascularEndothelialCells
CatalogNo.MK-6058
SuggestedMedium: CompleteMonkeyEndothelialCell Medium–125ml
CatalogNo.MK1168
(1-3weeks)
ProductDescription
CynomolgusMonkeyPrimaryThyroidMicrovascularEndothelialCellsfromCellBIOLOGicsareisolatedfromCynomolgusmonkeythyroidtissue.CynomolgusMonkeyPrimaryThyroidMicrovascularEndothelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
CynomolgusMonkeyPrimaryThyroidMicrovascularEndothelialCellsfromCellBiologicsdisplaytypicalcobblestonewithlargedarknucleiappearanceunderlightmicroscopy.CellsaretestedforexpressionofendothelialcellMarkerusingantibodyCD31(CatalogNo.ab9498,Abcam),VE-cadherinAntibody(CatalogNo.sc-28644,SantaCruz),orVE-cadherin(CatalogNo.ALX-210-232-C100,EnzoLifeSciences,Inc.)byimmunofluorescencestainingorFACS.Cellsarenegativeforbacteria,yeast,fungiandmycoplasma.CellscanbeexpandedonaplatereadyforexperimentsunderthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
CynomolgusMonkeyPrimaryThyroidMicrovascularEndothelialCellscanbeusedinassaysofcelltocelladhesion,migration,vasculartubeformation.StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen (-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest. Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
CynomolgusMonkeyPrimaryThyroidMicrovascularEndothelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughMonkeyPrimaryThyroidMicrovascularEndothelialCellsareisolatedfromnormalmonkeytissue,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
