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当前位置: 首页 > 产品中心 > acid_base_buffer_solution > 细胞生物学/食蟹猴原代肾小球内皮细胞/MK-6014G/
商品详细细胞生物学/食蟹猴原代肾小球内皮细胞/MK-6014G/
细胞生物学/食蟹猴原代肾小球内皮细胞/MK-6014G/
细胞生物学/食蟹猴原代肾小球内皮细胞/MK-6014G/
商品编号: MK-6014G
品牌: cellbiologics
市场价: ¥13000.00
美元价: 7800.00
产地: 美国(厂家直采)
公司:
产品分类: 酸碱缓冲液
公司分类: acid_base_buffer_solution
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Cynomolgus Monkey Primary Kidney Glomerular Endothelial Cells

Catalog No. MK-6014G

Suggested Medium: Complete Monkey Endothelial Cell  Medium – 125 ml

Catalog No. MK1168

                                                           

Product Description

Cynomolgus Monkey Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from Cynomolgus monkey kidney glomerular tissue. Cynomolgus Monkey Primary Kidney Glomerular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.

Product Testing

Cynomolgus Monkey Primary Kidney Glomerular Endothelial Cells from Cell Biologics display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody CD31 (Catalog No. ab9498, Abcam), VE-cadherin Antibody (Catalog No. sc-28644, Santa Cruz), or VE-cadherin (Catalog No. ALX-210-232-C100, Enzo Life Sciences, Inc.) by immunofluorescence staining or FACS. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded on a plate ready for experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Laboratory Applications

Cynomolgus Monkey Primary Kidney Glomerular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage of Cell Biologics’ Products

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen(-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.

Authorized Uses of Cell Biologics’ Products

Cynomolgus Monkey Primary Kidney Glomerular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.

Disclaimer

Appropriate safety procedures should always be used with this material. Although Cynomolgus Monkey Primary Kidney Glomerular Endothelial Cells are isolated from normal monkey tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Warranty and Liability

Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.

品牌介绍
Cell Biologics细胞生物制剂公司总部位于美国芝加哥,是全球独家提供Jackson Lab基因敲除小鼠原代细胞的专业公司,产品已获美国农业部SPF认证,并经中国进出口商品检验检疫局检验准许进口 自 成立以来,Cell Biologics已开发出13个品系1,000多种可直接使用的JacksonLab基因敲除小鼠、正常野生型小鼠、大鼠原代细胞,必将填补细胞生物学 界研究的空白。另外,Cell Biologics拥有AAALAC认证的动物实验室,提供JacksonLab与国外其他著名实验室所有允许商用的基因敲除小鼠原代细胞分离服务
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