CynomolgusMonkeyPrimaryCardiacMicrovascularEndothelialCells

CatalogNo.MK-6024

SuggestedMedium: CompleteMonkeyEndothelialCell  Medium–125ml

CatalogNo.MK1168

ProductDescription

CynomolgusMonkeyPrimaryCardiacMicrovascularEndothelialCellsfromCellBIOLOGicsareisolatedfromCynomolgusmonkeyhearttissue.MonkeyPrimaryCardiacMicrovascularEndothelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.

ProductTesting

CynomolgusMonkeyPrimaryCardiacMicrovascularEndothelialCellsfromCellBiologicsdisplaytypicalcobblestonewithlargedarknucleiappearanceunderlightmicroscopy.CellsaretestedforexpressionofendothelialcellMarkerusingantibodyCD31(CatalogNo.ab9498,Abcam),VE-cadherinAntibody(CatalogNo.sc-28644,SantaCruz),orVE-cadherin(CatalogNo.ALX-210-232-C100,EnzoLifeSciences,Inc.)byimmunofluorescencestainingorFACS.Cellsarenegativeforbacteria,yeast,fungiandmycoplasma.CellscanbeexpandedonaplatereadyforexperimentsunderthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.

LaboratoryApplications

CynomolgusMonkeyPrimaryCardiacMicrovascularEndothelialCellscanbeusedinassaysofcelltocelladhesion,migration,vasculartubeformation.StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.

StorageofCellBiologics’Products

CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest. Nevercanprimarycellsbekeptat-20°C.

AuthorizedUsesofCellBiologics’Products

CynomolgusMonkeyPrimaryCardiacMicrovascularEndothelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.AlthoughMonkeyPrimaryCardiacMicrovascularEndothelialCellsareisolatedfromnormalmonkeytissue,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

WarrantyandLiABIlity

CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.