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Cell Biologics/C57BL/6 Mouse Bone Marrow Endothelial Progenitor Cells/C57-6031/
C57BL/6MouseBoneMarrowEndothelialProgenitorCells
Cataloguenumber:C57-6031
SuggestedMedium:EndothelialCellMedium/wKit-500ml
CatalogNo.M1168
ProductDescription
C57BL/6MouseBoneMarrowEndothelialProgenitorCellsarederivedfromthetibiasandfemursofpathogen-freelaboratoryadultmice.C57BL/6MouseBoneMarrowEndothelialProgenitorCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedsolutionfor0.5hourandincubatedinCellBIOLOGics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106cellspermlandaredeliveredfrozen.
ProductTesting
C57BL/6MouseBoneMarrow Endothelial ProgenitorCellsaretestedforexpressionofMarkersusingfluorescentantibodies,Sca-1,Flk-1,orTie-2.ThecellswerealsotestedforuptakeofDil-Ac-LDL,afunctionalmarkerforendothelialcells.C57BL/6MouseBoneMarrow Endothelial ProgenitorCellsarenegativeforbacteria,yeast,fungiandmycoplasma,andcanbeexpandedfor3-6passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
Standardbiochemicalproceduresperformedwithprogenitorcellculturesincludecelladhesion,migration,RT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstainingorimmunofluorescentflowcytometryorgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice. Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Live-cellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
C57BL/6MouseBoneMarrowEndothelialProgenitorCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures. TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent. CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
AlthoughC57BL/6MouseBoneMarrow Endothelial ProgenitorCellsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
