iNOSKnockoutMousePrimaryEmbryonicFibroblasts
CatalogNo. iNOS-3028
SuggestedMedium:M2267MouseFibroblastMedium/wKit–500ML
ProductDescription
MousePrimaryEmbryonicFibroblastsfromCellBIOLOGicsareisolatedfromtissueofpathogen-freelaboratorymice.MousePrimaryEmbryonicFibroblastsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor0.5hourandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded. Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106cellspermlandisdeliveredfrozen.MousePrimaryEmbryonicFibroblastsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
StandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstainingorflowcytometry.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice. Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Livecellshipmentisalsoavailableonrequest.
AuthorizedUsesofCellBiologics’Products
MousePrimaryEmbryonicFibroblastsfromCellBiologicsaredistributedforresearchpurposesonly. Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures. TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent. CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaim
AlthoughMousePrimaryEmbryonicFibroblastsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcaution,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CELLBIOLOGICS’ guarantee applies only toyour purchase ofCELLBIOLOGICS'cellswithCELLBIOLOGICS’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCELLBIOLOGICS’onlineprotocolswithin35daysfromthedateofproductdelivery.
