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Cell Biologics/iPS-Derived Endothelial Cell Differentiated Medium/M6861/
iPS-DerivedEndothelialCellDifferentiatedMedium
CatalogNumber:M6861
Description
iPS-DerivedEndothelialCellsDifferentiatedMediadesignedforthedifferentiationofiPSintoendothelialcells.Mediumcontainsgrowthfactorsandsupplements.Itisformulatedforusewith5%CO2and95%airinahumidifiedincubator.Themediumconsistsof500mlofbasalmedium(containingessentialandnon-essentialaminoacids,vitamins,organicandinorganiccompounds,hormones,growthfactors,traceminerals),supplementedwithcellgrowthsupplement,antibiotics,andfetalbovineserum.
CellCultureMediumandSupplementsinclude:
- 125MLHam'sF-12NutrientMix
- 375ML IMDM
- 2.5MLN2-SUPPLEMENT
- 5.0MLB-27SupplementMinus
- 2.5ML10%BSA
- 0.5MLMTG(1-Thioglycerol,0.45M,1000x)
- 0.5MLAscorbicacid(50mg/ml,1000x)
*Beforeuse,addgrowthfactorsinto100mlbasalmediumwithsupplementsasfollowing:
- 0.1MLVEGF
- 20ul FGF-2
- 4ulBMP-4
StorageCondition
Store500mlbasalmediumat2-6°C.Storecellgrowthfactors,supplements,fetalbovineserum(FBS)at-20°C.ThecompletecellculturemediumwithSupplementscanbekeptin4°Cfor2-4weeks.Protectfromlight.
Toassuresterility,thepreparedmediumshouldbesterilizedbyfiltrationthrougha0.2µmfilter.
Shipping
Icepackanddryice.
AuthorizedUsesofCellBIOLOGics’Products
CompleteCellCultureMediafromCellBiologicsaredistributedforinvitroresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’CellsorProductsfromthepurchasertoothermarkets,organizations,orindividualsisprohibitedbyCellBiologics.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.