CompleteMouseEmbryonicStemCellMedium
CatalogNumber:M6822
Description
MouseEmbryonicStemCellMediumisacompletemediumdesignedforthecultureofmouseembryonicstemcellsandmouseinducedpluripotentstemcells.Itwastestedandoptimizedwithmouseembryonicstemcell(ESC)andinducedpluripotentstemcell(iPSC)growthandproliferationinvitro.Itisformulatedforusewith5%CO2and95%airinahumidifiedincubator.Themediumconsistsof500mlofbasalmedium(containingessentialandnon-essentialaminoacids,vitamins,organicandinorganiccompounds,hormones,growthfactors,traceminerals),supplementedwithfibroblastcellgrowthsupplement,antibiotics,andfetalbovineserum.15%FetalBovineSerum(germlinetransmission-tested)containsinthemedium.
CellCultureMediumSupplementsinclude:
-75.0MLFBS
-5.0MLGlutaMAX™Supplement
-5.0MLPenicillin-Streptomycin
-1.0ML2-Mercaptoethanol
-0.1MLLIF
*Beforeuse,addSupplementsinto410DMEMbasalmedium.
StorageCondition
Store500mlbasalmediumat2-6°C.Storecellgrowthfactors,supplements,fetalbovineserum(FBS)andantibioticsat-20°C.ThecompletecellculturemediumwithSupplementscanbekeptin4°Cfortwomonths.Protectfromlight.
Toassuresterility,thepreparedmediumshouldbesterilizedbyfiltrationthrougha0.2µmfilter.
Shipping
Icepackanddryice.
AuthorizedUsesofCellBIOLOGics’Products
CompleteCellCultureMediafromCellBiologicsaredistributedforinvitroresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’CellsorProductsfromthepurchasertoothermarkets,organizations,orindividualsisprohibitedbyCellBiologics.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
