CompleteEmbryonicFibroblastMedium

CatalogNumber:M6821

Description

EmbryonicFibroblastMediumisacompletemediumdesignedforthecultureofhumanandanimalembryonicfibroblasts.Itwastestedandoptimizedwithhuman,mouse,andratembryonicfibroblastgrowthandproliferationinvitro.Itisformulatedforusewith5%CO2and95%airinahumidifiedincubator.Themediumconsistsof500mlofbasalmedium(containingessentialandnon-essentialaminoacids,vitamins,organicandinorganiccompounds,hormones,growthfactors,traceminerals),supplementedwithfibroblastcellgrowthsupplement,antibiotics,andfetalbovineserum.

CellCultureMediumSupplementsinclude:

-50.0MLFBS

-5.0MLNEAA(MEMNon-EssentialAminoAcidsSolution)

-5.0MLL-Glutamine

-5.0MLPenicillin-Streptomycin

*Beforeuse,addSupplementsinto435MLDMEMbasalmedium.Forhumanpluripotentstemcellculture,thePen/Stepcouldbeeliminated.

StorageCondition

Store500mlbasalmediumat2-6°C.Storecellgrowthfactors,supplements,fetalbovineserum(FBS)andantibioticsat-20°C.ThecompletecellculturemediumwithSupplementscanbekeptin4°Cfortwomonths.Protectfromlight.

Toassuresterility,thepreparedmediumshouldbesterilizedbyfiltrationthrougha0.2µmfilter.

Shipping

Icepackanddryice.

AuthorizedUsesofCellBIOLOGics’Products

CompleteCellCultureMediafromCellBiologicsaredistributedforinvitroresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’CellsorProductsfromthepurchasertoothermarkets,organizations,orindividualsisprohibitedbyCellBiologics.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.