NOX2/Gp91(phox)KnockoutMousePrimaryEmbryonicFibroblasts
CatalogNo. Gp-6028
SuggestedMedium: FibroblastMedium/wKit-500ml
CatalogNo. M2267
ProductDescription
NOX2/Gp91(phox)KOMousePrimaryEmbryonicFibroblastsfrom CellBIOLOGics areisolatedfromembryonictissueofpathogen-freelaboratorymice.CellsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded. Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106 cellspermlandisdeliveredfrozen.
ProductTesting
NOX2/Gp91(phox) KO MousePrimaryEmbryonicFibroblastsaretestedforexpressionofMarkerusingtheantibodyofanti-FSP1/S100A4(MilliporeUSA)byimmunofluorescencestaining.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
NOX2/Gp91(phox) KOMousePrimaryEmbryonicFibroblastscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesof CellBiologics Products
NOX2/Gp91(phox) KOMousePrimaryEmbryonicFibroblastsfrom CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,for invitro diagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
