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Cell Biologics/Mouse Tumor-Associated Endothelial Cells (Mouse Colon Cancer Origin, HCT116)/MC-6203h/
MouseTumor-AssociatedEndothelialCells(MouseColonCancerOrigin,MTCLECH)
CatalogNo.MC-6203h
SuggestedMedium: EndothelialCellMedium/wKit–500ml
CatalogNo.M1168
ProductDescription
MouseTumor-AssociatedEndothelialCells(MouseColonCancerOrigin,MTCLECH)from CellBIOLOGics wereisolatedfrompathogen-freelaboratorynudemice.MTCLECHfrom CellBiologics wereisolatedfromhumanxenograftofpathogen-freelaboratorynudemice.MouseColonadenocarcinomaepithelialcells(HCT116)weresubcutaneouslyinjectedintothenudemicefor4-6weeksandthetumortissueswerethenharvestedforcellculture. MTCLECH wereculturedinaT25tissuecultureflaskpre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’ CultureCompleteGrowthMediumgenerallyfor14-35days.Cellsweredetachedfromflasksandimmediatelycryo-preservedinvials.Cellswillbedeliveredinafrozenvialandeachvialcontains0.5x106 cells/ml.Themethodweusetoisolateprimarytumor-associatedendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
MTCLECH aretestedforexpressionofMarkersusingantibody,Ve-Cadherin(CD144,VE-CadherinAntibody,C-19,sc6458SantaCruz);orCD31/PECAM-1(PurifiedRatAnti-MouseCD31,CatalogNo.553370,BD)byimmunofluorescencestainingorFACS. MTCLECH arenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-6passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
MTCLECH canbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,ortransendothelialresistance(TER).StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometryorgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.
Primarycellsshouldneverbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
MTCLECH from CellBiologics aredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
