MouseTumor-AssociatedEndothelialCells(MouseBreastCancerOrigin,MTMMECM)
CatalogNo.MC-6020m
SuggestedMedium: EndothelialCellMedium/wKit–500ml
CatalogNo.M1168
ProductDescription
MouseTumor-AssociatedEndothelialCells(MouseBreastCancerOrigin,MTMMECM)from CellBIOLOGics wereisolatedfrompathogen-freelaboratorynudemice.MTMMECMfrom CellBiologics wereisolatedfromhumanxenograftofpathogen-freelaboratorynudemice.Mousebreastadenocarcinomaepithelialcells(4T1)weresubcutaneouslyinjectedintothenudemicefor4-6weeksandthetumortissueswerethenharvestedforcellculture.MTMMECMwereculturedinaT25tissuecultureflaskpre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’ CultureCompleteGrowthMediumgenerallyfor14-35days.Cellsweredetachedfromflasksandimmediatelycryo-preservedinvials.Cellswillbedeliveredinafrozenvialandeachvialcontains0.5x106 cells/ml.Themethodweusetoisolateprimarytumor-associatedendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
MTMMECMaretestedforexpressionofMarkersusingantibody,Ve-Cadherin(CD144,VE-CadherinAntibody,C-19,sc6458SantaCruz);orCD31/PECAM-1(PurifiedRatAnti-MouseCD31,CatalogNo.553370,BD)byimmunofluorescencestainingorFACS.MTMMECMarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-6passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
MTMMECMcanbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,ortransendothelialresistance(TER).StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometryorgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.
Primarycellsshouldneverbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
MTMMECMfrom CellBiologics aredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
