MouseTumor-AssociatedEndothelialCells(HumanCervicalCancerOrigin,MTCVECH)
CatalogNo.MC-6236
SuggestedMedium: EndothelialCellMedium/wKit–500ml
CatalogNo.M1168
ProductDescription
MouseTumor-AssociatedEndothelialCells(HumanCervicalCancerOrigin,MTCVECH)fromCellBIOLOGicswereisolatedfrompathogen-freelaboratorynudemice.MTCVECHfromCellBiologicswereisolatedfromhumanxenograftofpathogen-freelaboratorynudemice.Humancervicaladenocarcinomaepithelialcells(ME-180)weresubcutaneouslyinjectedintothenudemicefor4-6weeksandthetumortissueswerethenharvestedforcellculture.MTCVECHwereculturedinaT25tissuecultureflaskpre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor14-35days.Cellsweredetachedfromflasksandimmediatelycryo-preservedinvials.Cellswillbedeliveredinafrozenvialandeachvialcontains0.5x106cells/ml.Themethodweusetoisolateprimarytumor-associatedendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
MTCVECHaretestedforexpressionofMarkersusingantibody,Ve-Cadherin(CD144,VE-CadherinAntibody,C-19,sc6458SantaCruz);orCD31/PECAM-1(PurifiedRatAnti-MouseCD31,CatalogNo.553370,BD)byimmunofluorescencestainingorFACS.MTCVECHarenegativeforbacteria,yeast,fungiandmycoplasma.Cellscanbeexpandedfor3-6passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
MTCVECHcanbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,ortransendothelialresistance(TER).StandardbiochemicalproceduresperformedwithendothelialcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometryorgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.
Primarycellsshouldneverbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
MTCVECHfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent. CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
