ZDFRatDiabeticCardiacFibroblasts
CatalogNo. RD-6049
SuggestedMedium: FibroblastMedium/wKit–500ml
CatalogNo. M2267
ProductDescription
ZDFRatDiabeticCardiacFibroblastsfrom CellBIOLOGics areisolatedfromhearttissueofmale,9weekoldlaboratoryZDFrat. CellsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics CultureCompleteGrowthMediumfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106 cellspermlandisdeliveredfrozen.
ProductTesting
ZDFRatDiabetic Cardiac Fibroblasts from CellBiologics aretestedfornegativeexpressionofvonWillebrandFactorExpression/FactorVIII,cytokeratin18,andalphasmoothmuscleactin.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
ZDFRatDiabetic Cardiac Fibroblasts canbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogenuntilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest. Primarycellscanneverbekeptat-20°C.
AuthorizedUsesof CellBiologics Products
ZDFRatDiabetic Cardiac Fibroblasts from CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,for invitro diagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics, withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
