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Cell Biologics/ZDF Rat Diabetic Esophageal Smooth Muscle Cells/RD-6089/
ZDFRatDiabetic Esophageal SmoothMuscleCells
CatalogNo. RD-6089
SuggestedMedium: SmoothMuscleCellMedium/wKit–500ml
CatalogNo. M2268
ProductDescription
ZDFRatDiabeticEsophagealSmoothMuscleCellsfrom CellBIOLOGics areisolatedfrom Esophageal tissueofmale,9weekoldlaboratoryZDFrat. CellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106 cellspermlandisdeliveredfrozen.
ProductTesting
ZDFRatDiabetic Esophageal SmoothMuscleCells arecharacterizedbyimmunofluorescentmethodwithantibodytoα-smoothmuscleactin. Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedonamultiwellcultureplatereadyforexperimentsunderthecellcultureconditionsspecifiedby CellBiologics (Cellsmaybeexpandedfor2or3passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics). Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
ZDFRatDiabetic Esophageal SmoothMuscleCells canbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen (-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.
AuthorizedUsesof CellBiologics’ Products
ZDFRatDiabetic Esophageal SmoothMuscleCells from CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics, withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
