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Cell Biologics/ZDF Rat Diabetic Spleen Epithelial Cells/RD-6045/ T75 F
ZDFRatDiabetic Spleen EpithelialCells
CatalogNo. RD-6045
SuggestedMedium: EpithelialCellMedium/wKit–500ml
CatalogNo. M6621
ProductDescription
ZDFRatDiabeticSpleenEpithelialCellsfrom CellBIOLOGics areisolatedfromspleentissueofmale,9weekoldlaboratoryZDFrat. CellsaregrowninT25tissuecultureflaskpre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’CellCultureMediumfor3-5days.Cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontains0.5x106 cellsandisdeliveredfrozen.Themethodweusetoisolateprimaryepithelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithEpCAM(CD326)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
ZDFRatDiabetic Spleen EpithelialCells arecharacterizedbyimmunofluorescentstainingwithantibodyofE-cadherin(Anti-E-CadherinAntibody,sc-8426,SantaCruz) orZO-1(Anti-ZO-1Antibody,MABT11,cloneR40.76,Millipore).Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
Standardbiochemicalproceduresperformedwithepithelialcellculturesincludetheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.
AuthorizedUsesof CellBiologics’ Products
ZDFRatDiabetic Spleen EpithelialCells from CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics, withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
