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Cell Biologics/ZDF Rat Diabetic Brain Microvascular Endothelial Cells/RD-6023/
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ZDFRatDiabetic BrainMicrovascularEndothelialCells
CatalogNo. RD-6023
SuggestedMedium: EndothelialCellMedium/wKit–500ml
CatalogNo. M1266
ProductDescription
ZDFRatDiabeticBrainMicrovascularEndothelialCellsfrom CellBIOLOGics areisolatedfrombraintissueofmale,9weekoldlaboratoryZDFrat. CellsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’CultureCompleteGrowthMediumfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106 cellspermlandisdeliveredfrozen.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1(CD31)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
ZDFRatDiabetic BrainMicrovascular EndothelialCellsaretestedforexpressionofMarkersusingantibody,PECAM-1Antibody(M-20,sc-1506,SantaCruz)orZO-1RabbitPolyclonalAntibody(CatalogNo.617300,LifeTechnologies)byimmunofluorescencestaining.CellsarealsotestedforuptakeofDil-Ac-LDL(CatalogNo.L-35353,Invitrogen),afunctionalmarkerforendothelialcells.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-5passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
ZDFRatDiabetic BrainMicrovascular EndothelialCellscanbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,transendothelialresistance(TER).StandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen (-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.
AuthorizedUsesof CellBiologics’ Products
ZDFRatDiabetic BrainMicrovascular EndothelialCellsfrom CellBiologics aredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics, withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswith CellBiologics’ MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
