Sprague-DawleyRatHepaticStellateCells
CatalogNo.RA-6242
SuggestedMedium:CompleteHepaticStellateCellMedium/wKit–500ml
CatalogNo.M5569
ProductDescription
Sprague-DawleyRatPrimaryHepaticStellateCellsfromCellBIOLOGicsareisolatedfromlivertissuesof4-6weekoldlaboratorySprague-Dawleyrat.CellsaregrownintissuecultureflasksandincubatedinCellBiologics’CellCultureMediumfor2-5days.Priortoshipping,cellsatpassage0aredetachedfromflasksandcryopreservedinvials.Eachvialcontains0.5x106cellspermlandisdeliveredfrozen.
ProductTesting
Sprague-DawleyRatPrimaryHepaticStellateCellsfromCellBiologicsaretestedforexpressionofMarkersusingantibodies,Desminorα-actinbyImmunofluorescenceStaining.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.CellscanbeexpandedonamultiwellcultureplatereadyforexperimentsunderthecellcultureconditionsspecifiedbyCellBiologics.HepaticStellateCellsarenotrecommendforexpandingorlongtermculturessincethesecellswoulddifferentiatetobecomefibroblat-likecellsimmediatelyafterplatingandtheywillnotproliferateinculture.
LaboratoryApplications
Sprague-DawleyRatPrimaryHepaticStellateCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogenuntilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.Primarycellscanneverbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
Sprague-DawleyRatPrimaryHepaticStellateCellsfromCellBiologicsaredistributedforinternalresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologics,withoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolution,forappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
