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Cell Biologics/Rat Primary Aortic Endothelial Cells- Wistar Rat/RA-6052WS/
RatPrimary Aortic EndothelialCells
CatalogNo. RA-6052WS
SuggestedMedium: EndothelialCellMedium/wKit–500ml
CatalogNo. M1266
ProductDescription
RatPrimaryAorticEndothelialCellsfrom CellBIOLOGics areisolatedfromaortaof1-day-oldneonatallaboratoryWistarrats.RatPrimaryAorticEndothelialCellsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106 cellspermlandisdeliveredfrozen.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1(CD31)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
RatPrimaryAorticEndothelialCellsaretestedforexpressionofMarkersusingantibody,PECAM-1Antibody(M-20,sc-1506,SantaCruz)orZO-1RabbitPolyclonalAntibody(CatalogNo.617300,LifeTechnologies)byimmunofluorescencestaining.RatPrimaryAorticEndothelialCellsarealsotestedforuptakeofDil-Ac-LDL(CatalogNo.L-35353,Invitrogen),afunctionalmarkerforendothelialcells.RatPrimaryAorticMicrovascularEndothelialCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
RatPrimaryAorticEndothelialCellscanbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,transendothelialresistance(TER).StandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen (-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesof CellBiologics’ Products
RatPrimaryAorticEndothelialCellsfrom CellBiologics aredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ cellswithCellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
