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Cell Biologics/Lewis Rat Primary Aortic Endothelial Cells/RA-6052LS/ T
LewisRatPrimaryAorticEndothelialCells
CatalogNo.RA-6052LS
SuggestedMedium:EndothelialCellMedium/wKit–500ml
CatalogNo.M1266
ProductDescription
LewisRatPrimaryAorticEndothelialCellsfromCellBIOLOGicsareisolatedfromaortaof6-8weekoldlaboratoryLewisrat.LewisRatPrimaryAorticEndothelialCellsaregrowninT75tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106cellspermlandisdeliveredfrozen.Themethodweusetoisolateprimaryendothelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.Thesecellsarepre-coatedwithPECAM-1(CD31)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
LewisRatPrimaryAorticEndothelialCellsaretestedforexpressionofMarkersusingantibody,PECAM-1Antibody(M-20,sc-1506,SantaCruz)orZO-1RabbitPolyclonalAntibody(CatalogNo.617300,LifeTechnologies)byimmunofluorescencestaining.LewisRatPrimaryAorticEndothelialCellsarealsotestedforuptakeofDil-Ac-LDL(CatalogNo.L-35353,Invitrogen),afunctionalmarkerforendothelialcells.LewisRatPrimaryAorticEndothelialCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2or1:3underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
LewisRatPrimaryAorticEndothelialCellscanbeusedinassaysofcell-celladhesion,migration,vasculartubeformation,transendothelialresistance(TER).StandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,westernblotting,immunoprecipitation,immunofluorescentstaining,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen
(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
LewisRatPrimaryAorticEndothelialCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
