RatPancreaticIslets

CatalogNo. RA-6232L(LiveCells); RA-6232F(FrozenVial)

SuggestedMedium:Pancreaticisletculturemedium/wKit–500ML

CatalogNo. M2269

ProductDescription

RatPancreaticIsletsfrom CellBIOLOGics areisolatedfromthepancreasof Sprague–Dawleyrat usingCollagenasePandpurifiedusingFicolldensitygrADIent.Theisolatedisletsareculturedin CellBiologics’ PancreaticIsletCultureMediumcontaining5%FBS.

ProductTesting

Rat PancreaticIsletsaretestedfortheirviABIlityusingFDA/PI,purityusingDithizonestaining,staticinsulinsecretioninresponseto14mMglucose.Uponrequest,dynamicinsulinsecretionandcalciumimaginginresponsetoglucoseandotherstimulatorscanbeperformed.

LaboratoryApplications

Rat PancreaticIsletscanbeusedfortheassayofisletfunction,drugtesting,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orothersfordesiredresearchapplications.

Storageof CellBiologics’ Products

CellBiologics shipsfreshisletcellsonateitherroomtemperatureor4°C.Onreceipt,immediatelytransferthecellsto37°Cand5%CO₂ incubator.

AuthorizedUsesof CellBiologics’ Products

Rat PancreaticIsletsfrom CellBiologics aredistributedforinternallaboratoryresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,notforinvitrodiagnosticproceduresorfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

WarrantyandLiability

CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ Cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.

PrimaryCellCultureProtocol

• Allcellcultureproceduresmustbeconductedinabio-safetycabinet.
• Anyandallmedia,supplements,andreagentsmustbesterilizedbyfiltrationthrougha0.2µmfilter.
• Useaseptictechniquetopreventmicrobialcontamination.
• Cryo-preservedcellsmustbestoredinliquidnitrogenorseededimmediatelyuponarrival.

Medium: 

• Reviewtheinformationprovidedonthe CellBiologics websiteaboutappropriateculturemedia(e.g.serumandothersupplements).Usepre-warmed(37°C)cellculturemedia(30-50ML)torecovercryopreservedcellsandwhenchangingmediaorsplittingcells.
• HandlingofArrivingLiveCells
• Whenyoureceivethelivecellsinatube,keepcellsin37°CCO₂ incubatorfor15min.
• Centrifugecellsat500rpmfor1minute.
• AspiratethesupernatantandresUSPendthecellpelletin6mlof CellBiologics’CellCulture Growthmedium(CatalogNo.M2269).
• Transfercellsfromavialtothecellcultureplate.
• Placeaplateinahumidified,5%-CO₂ incubatorat37°Cuntilexperiments.
• Cellscanbeculturedfor3-7days.
• Changefreshcellculturemediumevery24-48hours.
• Cellsshouldbecheckeddailyunderamicroscopytoverifyappropriatecellmorphology.