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Cell Biologics/Mouse Primary Diabetic Pulmonary Artery Smooth Muscle Cells/MD-6083/
MousePrimaryDiabeticPulmonaryArterySmoothMuscleCells
CatalogNo. MD-6083
SuggestedMedium: SmoothMuscleCellMedium/wKit–500ML
CatalogNo. M2268
ProductDescription
MousePrimaryDiabeticPulmonaryArterySmoothMuscleCellsfrom CellBIOLOGics areisolatedfromthepulmonaryarteryofDiabetic(db/db)mice(8weeks).MousePrimaryDiabeticPulmonaryArterySmoothMuscleCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedin CellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Priortoshipping,cellsatpassage1aredetachedfromthecultureflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106 cellspermlandisdeliveredfrozen.
ProductTesting
MousePrimaryDiabeticPulmonaryArterySmoothMuscleCellsfrom CellBiologics arecharacterizedbyimmunofluorescentmethodwithantibodiestoα-smoothmuscleactin.MousePrimaryDiabeticPulmonaryArterySmoothMuscleCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedonamultiwellcultureplatereadyforexperiments(orcellsmaybeexpandedfor2-4passagesatasplitratioof1:2)underthecellcultureconditionsspecifiedby CellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
MousePrimaryDiabeticPulmonaryArterySmoothMuscleCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
Storageof CellBiologics’ Products
CellBiologics shipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.Primarycellsshouldneverbekeptat-20°C.
AuthorizedUsesof CellBiologics’ Products
MousePrimaryDiabeticPulmonaryArterySmoothMuscleCellsfrom CellBiologics aredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.Transferorresaleofany CellBiologics’ cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedby CellBiologics withoutthecompany’swrittenconsent. CellBiologics’ TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineat http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’ guaranteeappliesonlytoyourpurchaseof CellBiologics’ Cellswith CellBiologics’ MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowing CellBiologics’ onlineprotocolswithin35daysfromthedateofproductdelivery.
