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Cell Biologics/Mouse Primary Diabetic Ovarian Smooth Muscle Cells/MD-6091/
MousePrimaryDiabeticOvarianSmoothMuscleCells
CatalogNo. MD-6091
SuggestedMedium: SmoothMuscleCellMedium/wKit–500ML
CatalogNo.M2268
ProductDescription
MousePrimaryDiabeticOvarianSmoothMuscleCellsfromCellBIOLOGicsareisolatedfromtheovarianofDiabetic(db/db)mice(8weeks).MousePrimaryDiabeticOvarianSmoothMuscleCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Priortoshipping,cellsatpassage1aredetachedfromthecultureflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106cellspermlandisdeliveredfrozen.
ProductTesting
MousePrimaryDiabeticOvarianSmoothMuscleCellsfromCellBiologicsarecharacterizedbyimmunofluorescentmethodwithantibodiestoα-smoothmuscleactin.MousePrimaryDiabeticOvarianSmoothMuscleCellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedonamultiwellcultureplatereadyforexperiments(orcellsmaybeexpandedfor2-4passagesatasplitratioof1:2)underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
MousePrimaryDiabeticOvarianSmoothMuscleCellscanbeusedinassaysofstandardbiochemicalproceduresperformedwithcellculturesincludeRT-PCR,Westernblotting,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Live-cellshipmentisalsoavailableonrequest.Primarycellsshouldneverbekeptat-20°C.
AuthorizedUsesofCellBiologics’Products
MousePrimaryDiabeticOvarianSmoothMuscleCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’CellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
