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Cell Biologics/Diabetic Mouse Alveolar Epithelial Cells/MD-6053/ T75 F
DiabeticMouse AlveolarEpithelialCells
CatalogNo. MD-6053
SuggestedMedium:EpithelialCellMedium/wKit–500ml
CatalogNo.M6621
ProductDescription
DiabeticMouseAlveolarEpithelialCellsfromCellBIOLOGicsareisolatedfromthelungofDiabetic(db/db)mice. DiabeticMouse AlveolarEpithelialCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBiologics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsatpassage3aredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast0.5x106cellsperml.Themethodweusetoisolateprimaryepithelialcellswasdevelopedbasedonacombinationofestablishedandourproprietarymethods.CellsareincubatedwithEpCAM-1(CD326)antibody,followingtheapplicationofmagneticbeadspre-coatedwithsecondaryantibody.
ProductTesting
DiabeticMouse AlveolarEpithelialCellsarecharacterizedbyimmunofluorescentstainingwithantibodiesofE-cadherin(Catalog610182,BD),ZO-1(Catalog617300,LifeTechnology)orSupernatant(T1alpha).T1alphawasidentifiedbyamonoclonalantibodypreviouslyshowntobespecificforanantigenexpressedbyalveolarepithelialtypeIcells.Cellsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor3-7passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
DiabeticMouse AlveolarEpithelialCellscanbeusedfortheassayofcell-cellinteraction,adhesion,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologics’Products
CellBiologicsshipsfrozencellsondryice.Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse.Livecellshipmentisalsoavailableonrequest.Primarycellsshouldneverbekeptat-20°C
AuthorizedUsesofCellBiologics’Products
DiabeticMouse AlveolarEpithelialCellsfromCellBiologicsaredistributedfor internal laboratoryresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,notforinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’CellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.