C57BL/6MousePancreaticIslets

CatalogNo. C57-6232L(LiveCells); C57-6232F(FrozenVial)

SuggestedMedium:Pancreaticisletculturemedium/wKit–500ML

CatalogNo.M2269

ProductDescription

C57BL/6MousePancreaticIsletsfromCellBIOLOGicsareisolatedfromthepancreasofC57BL/6miceusingCollagenasePandpurifiedusingFicolldensitygrADIent.TheisolatedisletsareculturedinCellBiologics’PancreaticIsletCultureMediumcontaining5%FBS.

ProductTesting

C57BL/6MousePancreaticIsletsaretestedfortheirviABIlityusingFDA/PI,purityusingDithizonestaining,staticinsulinsecretioninresponseto14mMglucose.Uponrequest,dynamicinsulinsecretionandcalciumimaginginresponsetoglucoseandotherstimulatorscanbeperformed.

LaboratoryApplications

C57BL/6MousePancreaticIsletscanbeusedfortheassayofisletfunction,drugtesting,PCR,Westernblot,immunoprecipitation,immunofluorescentflowcytometry,orothersfordesiredresearchapplications.

StorageofCellBiologics’Products

CellBiologicsshipsfreshisletcellsonateitherroomtemperatureor4°C.Onreceipt,immediatelytransferthecellsto37°Cand5%CO₂incubator.

AuthorizedUsesofCellBiologics’Products

C57BL/6MousePancreaticIsletsfromCellBiologicsaredistributedforinternallaboratoryresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,notforinvitrodiagnosticproceduresorfortherapeuticprocedures.TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent.CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.

Disclaimer

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.InvestigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallprimarycellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.TheentiretextofdiscussingBiosafetyinMicrobiologicalandBiomedicalLaboratories,5thed.isavailableonlineathttp://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

WarrantyandLiability

CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’CellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.

PrimaryCellCultureProtocol

• Allcellcultureproceduresmustbeconductedinabio-safetycabinet.
• Anyandallmedia,supplements,andreagentsmustbesterilizedbyfiltrationthrougha0.2µmfilter.
• Useaseptictechniquetopreventmicrobialcontamination.
• Cryo-preservedcellsmustbestoredinliquidnitrogenorseededimmediatelyuponarrival.

Medium: 

• ReviewtheinformationprovidedontheCellBiologicswebsiteaboutappropriateculturemedia(e.g.serumandothersupplements).Usepre-warmed(37°C)cellculturemedia(30-50ML)torecovercryopreservedcellsandwhenchangingmediaorsplittingcells.
• HandlingofArrivingLiveCells
• Whenyoureceivethelivecellsinatube,keepcellsin37°CCO₂incubatorfor15min.
• Centrifugecellsat500rpmfor1minute.
• AspiratethesupernatantandresUSPendthecellpelletin6mlofCellBiologics’CellCulture Growthmedium(CatalogNo.M2269).
• Transfercellsfromavialtothecellcultureplate.
• Placeaplateinahumidified,5%-CO₂incubatorat37°Cuntilexperiments.
• Cellscanbeculturedfor3-7days.
• Changefreshcellculturemediumevery24-48hours.
• Cellsshouldbecheckeddailyunderamicroscopytoverifyappropriatecellmorphology.