CD1MouseBoneMarrowEndothelialProgenitorCells
Cataloguenumber:CD-1031
SuggestedMedium:EndothelialCellMedium/wKit-500ml
CatalogNo.M1168
ProductDescription
CD1MouseBoneMarrowEndothelialProgenitorCellsarederivedfromthetibiasandfemursofpathogen-freelaboratoryadultmice.CD1MouseBoneMarrowEndothelialProgenitorCellsaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedsolutionfor0.5hourandincubatedinCellBIOLOGics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106cellspermlandaredeliveredfrozen.
ProductTesting
CD1MouseBoneMarrow Endothelial ProgenitorCellsaretestedforexpressionofMarkersusingfluorescentantibodiesCD31andSca-1orTie-2.CD1MouseBoneMarrow Endothelial ProgenitorCellsarenegativeforbacteria,yeast,fungiandmycoplasma,andcanbeexpandedfor3-6passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
Standardbiochemicalproceduresperformedwithprogenitorcellculturesincludecelladhesion,migration,RT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstainingorimmunofluorescentflowcytometryorgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice. Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Live-cellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
CD1MouseBoneMarrowEndothelialProgenitorCellsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures. TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent. CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
AlthoughCD1MouseBoneMarrow Endothelial ProgenitorCellsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
