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Cell Biologics/CD-1 Mouse Bone Marrow Mesenchymal Stem Cells/CD-1043/
CD-1MouseBoneMarrowMesenchymalStemCells(MBMMSC)
Cataloguenumber:CD-1043
SuggestedMedium:MesenchymalCellMedium/wKit–500ML
CatalogNo.M5566
ProductDescription
CD1MouseBoneMarrowMesenchymalStemCellsarederivedfromthetibiasandfemursofpathogen-freelaboratoryadultmice. TheyaregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedcoatingsolutionfor2minandincubatedinCellBIOLOGicsCultureCompleteGrowthMediumfor7-15days.Culturesarethenexpanded. Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials. Eachvialcontainsatleast2x106cellspermlandisdeliveredfrozen.MBMMSCsarenegativeforbacteria,yeast,fungi,andmycoplasma.Cellscanbeexpandedfor2or3passagesatasplitratioof1:2underthecellcultureconditionsspecifiedbyCellBiologics.Repeatedfreezingandthawingofcellsisnotrecommended.
MBMMSCsaretestedforexpressionofMarkersusingantibodies,CD44,Sca-1andCD29byflowcytometry.
LaboratoryApplications
MBMMSCscanbeusedinstandardbiochemicalproceduresincludePCR,Westernblotting,immunoprecipitation,orcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice. Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Live-cellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
MBMMSCsfromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticprocedures,orfortherapeuticprocedures. TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent. CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
AlthoughMBMMSCsareisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefromCellBiologicswithcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
