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Cell Biologics/C57BL/6 Mouse Primary Bone Marrow-Derived Endothelial Cells/C57-6031/
C57BL/6MousePrimaryBoneMarrow-DerivedEndothelialCells
Cataloguenumber:C57-6031
SuggestedMedium:EndothelialCellMedium/wKit-500ml
CatalogNo.M1168
ProductDescription
C57BL/6MousePrimaryBoneMarrow-DerivedEndothelialCells arederivedfromthetibiasandfemursofpathogen-freelaboratoryadultmice.C57BL/6Mouse Primary BoneMarrow-DerivedEndothelialCells aregrowninT25tissuecultureflaskspre-coatedwithgelatin-basedsolutionfor0.5hourandincubatedinCellBIOLOGics’CultureCompleteGrowthMediumgenerallyfor3-7days.Culturesarethenexpanded.Priortoshipping,cellsaredetachedfromflasksandimmediatelycryo-preservedinvials.Eachvialcontainsatleast1x106cellspermlandaredeliveredfrozen.
ProductTesting
C57BL/6Mouse Primary BoneMarrow-DerivedEndothelialCellsaretestedforexpressionofMarkersusingfluorescentantibodiesofCD31andSca-1orTie-2.C57BL/6Mouse Primary BoneMarrow-DerivedEndothelialCells arenegativeforbacteria,yeast,fungiandmycoplasma,andcanbeexpandedfor3-6passagesatasplitratioof1:2underthecellcultureconditionsspecifiedby CellBiologics. Repeatedfreezingandthawingofcellsisnotrecommended.
LaboratoryApplications
StandardbiochemicalproceduresperformedwithProgenitorcellculturesincludecelladhesion,migration,RT-PCR,Westernblotting,immunoprecipitation,immunofluorescentstainingorimmunofluorescentflowcytometryorgeneratingcellderivativesfordesiredresearchapplications.
StorageofCellBiologicsProducts
CellBiologicsshipsfrozencellsondryice. Onreceipt,immediatelytransferfrozencellstoliquidnitrogen(-180°C)untilreadyforexperimentaluse. Live-cellshipmentisalsoavailableonrequest.
Nevercanprimarycellsbekeptat-20°C.
AuthorizedUsesofCellBiologicsProducts
C57BL/6MouseBoneMarrow-DerivedEndothelialCells fromCellBiologicsaredistributedforresearchpurposesonly.Ourproductsarenotauthorizedforhumanuse,forinvitrodiagnosticproceduresorfortherapeuticprocedures. TransferorresaleofanyCellBiologics’cellsorproductsfromthepurchasertoothermarkets,organizationsorindividualsisprohibitedbyCellBiologicswithoutthecompany’swrittenconsent. CellBiologics’TermsandConditionsmustbeacceptedbeforesubmittinganorder.
Disclaimer
AlthoughC57BL/6Mouse Primary BoneMarrow-DerivedEndothelialCells areisolatedfromlaboratorymicetestingpathogen-free,investigatorsshouldhandlethecellsthattheyreceivefrom CellBiologics withcautionandtreatallanimalcellsaspotentialpathogens,sincenotestprocedurecancompletelyguaranteetheabsenceofinfectiousagents.
WarrantyandLiABIlity
CellBiologics’guaranteeappliesonlytoyourpurchaseofCellBiologics’cellswithCellBiologics’MediaandCoatingSolutionforappropriatecellcultureandcelltestingfollowingCellBiologics’onlineprotocolswithin35daysfromthedateofproductdelivery.
